The Association of age at Menarche and Adult Height With Mammographic Density in the International Consortium of Mammographic Density

Sarah V. Ward; Anya Burton; Rulla M. Tamimi; Ana Pereira; Maria Luisa Garmendia; Marina Pollan; Norman Boyd; Isabel dos-Santos-Silva; Gertraud Maskarinec; Beatriz Perez-Gomez; Celine Vachon; Hui Miao; Martín Lajous; Ruy López-Ridaura; Kimberly Bertrand; Ava Kwong; Giske Ursin; Eunjung Lee; Huiyan Ma; Sarah Vinnicombe; Sue Moss; Steve Allen; Rose Ndumia; Sudhir Vinayak; Soo-Hwang Teo; Shivaani Mariapun; Beata Peplonska; Agnieszka Bukowska-Damska; Chisato Nagata; John Hopper; Graham Giles; Vahit Ozmen; Mustafa Erkin Aribal; Joachim Schüz; Carla H. Van Gils; Johanna O. P. Wanders; Reza Sirous; Mehri Sirous; John Hipwell; Jisun Kim; Jong Won Lee; Caroline Dickens; Mikael Hartman; Kee-Seng Chia; Christopher Scott; Anna M. Chiarelli; Linda Linton; Anath Arzee Flugelman; Dorria Salem; Rasha Kamal; Valerie McCormack; Jennifer Stone

Disclosures

Breast Cancer Res. 2022;24(49)

Results

Participant Characteristics

Characteristics of the participants, overall and by ethnic group, are shown in Table 1. The mean age at mammogram was 52.7 years (standard deviation (SD) = 8.2 years), which was relatively consistent across all groups except the Mestizo women, who were, on average, approximately 10 years younger. The mean age at menarche was also similar across all ethnic groups and, on average, occurred at a 13.2 years (SD = 1.7 years). Height varied slightly across the ethnic groups, with average height notably shorter in the East Asian, South Asian and Mestizo groups. There were more post-menopausal than pre-menopausal women in all ethnic groups except the Mestizo group, consistent with their younger age, and there were substantially more parous (90%) than nulliparous (10%) women.

Age at Menarche

Forest plots depicting population group-specific associations for age at menarche with each of the MD measures, along with meta-analyses results for overall associations, are presented in Figures 1 (PD) and 2 (DA). Overall, a small positive association was observed between the square-root change in both PD (β = 0.02, 95% CI 0.00, 0.03) and DA (β = 0.03, 95% CI 0.01, 0.05) with each yearly increase in age at menarche. Results were highly consistent across all studies for √PD (I ² = 12.1%) but less so for √DA (I ² = 32.4%), although both were considered to have low heterogeneity overall.

Pooled analyses of all participants (Table 2) showed very similar overall effect estimates to the meta-analyses. Later age at menarche was associated with increased √PD in all women (β = 0.057, SE = 0.008, P < 0.001). Results were attenuated when adjusted for BMI (β = 0.023, SE = 0.008, P = 0.003), similar to meta-analysis estimates. Adjustment for height did not alter the effect estimate substantially. Stratified analyses showed that this association was primarily driven by women with a BMI under 25 kg/m² and by parous women. The association was present at both pre- and post-menopausal ages.

Similar results were observed for DA; later age at menarche was also associated with an increase in √DA for all women (β = 0.032, SE = 0.010, P = 0.002). The association with √DA was also more evident in parous women and women with lower BMI (BMI ≤ 25: P < 0.001) and did not differ by menopausal status. Results were generally unaffected after adjusting for age at first birth or age at menopause.

Height

Population group-specific and meta-analyses results for the association of MD measures with height are shown in Figures 3 (PD) and 4 (DA). Overall, there was no strong evidence of an association between √PD and height across studies (β = − 0.04 per 10 cm height increment, 95% CI − 0.08, 0.01), but there was evidence of an overall increase for √DA per 10 cm increase in height (β = 0.08, 95% CI 0.02, 0.14). Heterogeneity across population groups was relatively low for each MD measure (√PD, I ² = 22.6%; √DA, I ² = 20.9%).

Pooled results for both measures of MD and their association with adult height are presented in Table 3. For height and PD, the association was positive without adjustment for BMI or when adjusted for the study-specific weight-for-height index (Additional file 1: Table S2), but inversely associated when adjusted for BMI. The latter appeared to reflect the strong inverse association of height and BMI in almost all population groups (Additional file 1: Table S1). In the stratified analyses (adjusted for BMI), the height-√PD associations were largely negative, regardless of parity or menopausal status. When subset by the method of height measurement, an association was only observed in those women who had self-reported their height.

Consistent with the meta-analysis, an increase was observed in √DA with increased height (β = 0.059, SE = 0.028, P = 0.034) and this association was slightly stronger without adjustment for BMI (β = 0.069, SE = 0.028, P = 0.012). When stratified by BMI category, women in all but the highest (> 30 kg/m²) categories had an increased √DA with increased height. When analysed by height ascertainment method, the height-√DA association was stronger when height was measured (β = 0.084, SE = 0.034, P = 0.015) as opposed to self-reported (β = 0.020, SE = 0.047, P = 0.676). The association with √DA was stronger in parous women, overall (β = 0.090, SE = 0.030, P = 0.002), and in both pre- (β = 0.099, SE = 0.047, P = 0.034) and post-menopausal parous women (β = 0.090, SE = 0.039, P = 0.019).

No differences in results were observed for any outcome measures when adjusted for age at first birth in parous women or age at menopause in the post-menopausal group.

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Breast Cancer Res. 2022;24(49) © 2022 BioMed Central, Ltd.
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Table 1. Characteristics of participants in the International Consortium on Mammographic Density
World Region^a:	All women (n = 10,681)	White (n = 4512)	Black (n = 938)	East Asian (n = 1837)	South Asian (n = 1133)	Mestizo (n = 333)	Hawaiian (n = 142)	Middle Eastern (n = 1786)
World Region^a:	n [column %] unless stated otherwise
Per cent density (cm²)
Median (IQR)	20 (11–31)	22 (16–35)	17 (10–26)	24 (16–35)	16 (8–25)	20 (13–27)	18 (9–32)	16 (8–24)
Dense area (cm²)
Median (IQR)	27 (16–41)	27 (17–42)	34 (20–49)	24 (15–35)	22 (12–35)	28 (18–42)	25 (12–38)	29 (16–43)
Age at mammogram (years)
Mean (SD)	52.7 (8.2)	53.8 (7.7)	52.4 (8.5)	52.1(8.5)	53.6 (7.2)	44.2 (6.4)	55.1 (7.5)	51.4 (8.5)
IQR	47–58	49–59	46–58	45–58	49–59	40–49	50–61	45–57
Age at menarche (yrs)^b
< 12.0	1470 [14]	838 [19]	80 [9]	166 [9]	153 [14]	77 [23]	20 [14]	136 [8]
12.0–12.9	2588 [24]	1031 [23]	116 [12]	437 [24]	300 [26]	87 [26]	56 [39]	561 [31]
13.0–13.9	2594 [24]	1185 [26]	167 [18]	350 [19]	283 [25]	73 [22]	0 [0]	536 [30)
14.0–14.9	2023 [19]	848 [19]	182 [19]	425 [23]	165 [15]	46 [14]	47 [33]	310 [17]
≥ 15.0	2006 [19]	610 [14]	393 [42]	459 [25]	232 [20]	50 [15]	19 [13]	243 [14]
Mean age (SD)	13.2 (1.7)	12.9 (1.6)	14.2 (2.1)	13.5 (1.8)	13.2 (1.7)	12.7 (1.7)	12.9 (1.8)	13.0 (1.4)
Height (cm)
< 155	2760 [26]	685 [15]	186 [20]	704 [38]	663 [59]	113 [34]	17 [12]	392 [22]
155–159.9	2609 [24]	905 [20]	211 [22]	604 [33]	268 [24]	125 [38]	15 [11]	481 [27]
160–164.9	2759 [26]	1299 [29]	287 [31]	395 [22]	136 [12]	70 [21]	42 [30]	530 [30]
> 165	2553 [24]	1623 [36]	254 [27]	134 [7]	66 [6]	25 [8]	68 [48]	383 [21]
Mean height (SD)	159.4 (7.2)	162.0 (6.9)	160.6 (7.6)	156.4 (5.6)	153.2 (6.8)	157.0 (5.3)	163.2 (6.0)	159.4 (6.3)
% of measured heights^c	66	57	39	57	85	98	0	100
BMI
≤ 20.0	642 (6)	208 (5)	9 (1)	284 (15)	95 (8)	3 (1)	2 (1)	41 (2)
20.1–25.0	3920 (37)	1787 (40)	171 (18)	1086 (59)	376 (33)	82 (25)	43 (30)	375 (21)
25.1–30.0	2342 (32)	1503 (33)	320 (34)	411 (22)	428 (38)	133 (40)	45 (32)	592 (33)
> 30.0	2687 (25)	1014 (22)	438 (47)	56 (3)	234 (21)	115 (35)	52 (37)	778 (44)
Mean BMI (SD)	27.0 (5.7)	26.7 (5.3)	30.3 (6.2)	23.2 (3.4)	26.3 (5.0)	28.7 (5.1)	28.9 (6.3)	29.6 (6.0)
Menopausal status
Pre-menopausal	4361 [41]	1761 [39]	328 [35]	799 [43]	324 [29]	261 [78]	35 [25]	853 [48]
Post-menopausal	6320 [59]	2751 [61]	610 [65]	1038 [57]	809 [71]	72 [22]	107 [75]	933 [52]
Ever used hormone therapy^d
Yes	2185 [25]	1387 [36]	133 [14]	384 [21]	82 [8]	27 [8]	78 [55]	94 [12]
No	6681 [75]	2456 [64]	802 [86]	1425 [79]	969 [92]	291 [92]	64 [45]	674 [88]
Parity
Nulliparous	1068 [10]	661 [15]	61 [7]	161 [9]	90 [8]	21 [6]	9 [6]	65 [4]
Parous	9613 [90]	3851 [85]	877 [94]	1,676 [91]	1,043 [92]	312 [94]	133 [94]	1721 [96]
Mean parity (SD)	2.6 (1.8)	2.4 (1.1)	3.3 (1.7)	2.5 (1.2)	3.9 (2.1)	2.9 (1.4)	3.3 (1.5)	3.6 (2.2)
Age at first birth (yrs)^b
Mean age (SD)	24.3 (5.1)	25.3 (4.7)	22.3 (4.8)	26.2 (4.2)	22.4 (6.2)	23.9 (5.0)	22.9 (4.1)	22.5 (5.2)
IQR	21–27.5	22–27.8	19–25	23–28.3	18–26	19.8–27	19–23	19–26

BMI body mass index, cm centimetres, IQR inter-quartile range, SD standard deviation, yrs years
^aPopulation groups and study source, by world region
White: White women in Australia (3 studies—Australian, Greek and Italian born), Canada, Netherlands, Norway, Poland, Spain, the UK (3 studies) and the USA (mainland: 3 studies, Hawaii: 1 study)
Black: Black women in Kenya, South Africa, the UK and the USA
East Asian: Chinese women in Hong Kong, Malaysia and Singapore; Japanese women in Japan, Korea and the USA (mainland: 1 study, Hawaii: 1 study)
South Asian: Malay women in Malaysia and Singapore; Indian women in India, Malaysia, Singapore and the UK
Mestizo: Mestizo women in Chile and Mexico
Hawaiian: Hawaiian women in USA (Hawaii: 1 study)
Middle Eastern: Egyptian women in Egypt; Arab women in Israel; Jewish women in Iran, Israel and Turkey
^bNote raw data recorded categorically by some studies and subsequently converted to an approximated numerical value
^cHeight data that were measured by the original study, as opposed to self-reported
^dAmong women with non-missing data. Missing data on hormone therapy were 17% overall, < 4% for black, Hawaiian, Mestizo and East Asian, 15% missing for white women and 57% for Middle Eastern. These women were included in a missing-hormone-therapy category in later regression models

Table 2. Association of per cent density and dense area with age at menarche in pooled ICMD studies
	No. of women	No. of measurements	Per cent density^a		Dense area^a,f
	No. of women	No. of measurements	Changes in square-root per cent density (SE)	P value	Changes in square-root dense area (SE)	P value
Age at menarche category (years)
< 12.0	1470	1722	0		0
12.0–12.9	2588	3036	0.006 (0.042)	0.889	− 0.032 (0.056)	0.575
13.0–13.9	2594	3067	0.064 (0.042)	0.130	0.053 (0.056)	0.346
14.0–14.9	2023	2397	0.057 (0.045)	0.207	0.034 (0.059)	0.569
≥ 15.0	2006	2364	0.128 (0.046)	0.005	0.166 (0.061)	0.007
Linear associations with a 1 year increase in age at menarche
All women—Model A^c
All, BMI and height adjusted	10,681	12,586	0.023 (0.008)	0.003	0.032 (0.010)	0.002
All, without BMI adjustment	10,681	12,586	0.057 (0.008)	< 0.001	0.036 (0.010)	0.001
All, without height adjustment	10,681	12,586	0.022 (0.008)	0.004	0.033 (0.010)	0.001
Subset by BMI group (kg/m²)
≤ 20.0	652	752	0.037 (0.032)	0.251		N/A^b
20.1–25.0	3915	4585	0.039 (0.013)	0.002	0.068 (0.014)	< 0.001
25.1–30.0	3427	4048	0.013 (0.014)	0.328	0.005 (0.018)	0.782
> 30.0	2687	3201	0.012 (0.016)	0.456	0.009 (0.023)	0.704
Pre-menopausal women—Model B^d
All	4349	5135	0.020 (0.013)	0.122	0.028 (0.017)	0.111
Subset by parity
Nulliparous women	483	558	− 0.044 (0.036)	0.226	0.009 (0.048)	0.847
Parous women	3866	4577	0.027 (0.013)	0.045	0.026 (0.018)	0.162
Parous women + age 1st birth adjustment	3866	4577	0.027 (0.013)	0.044	0.025 (0.018)	0.165
Post-menopausal women—Model C^d
All	5868	6902	0.022 (0.010)	0.029	0.029 (0.013)	0.029
All + age at menopause adjustment	5868	6902	0.022 (0.010)	0.031	0.029 (0.013)	0.031
Subset by parity
Nulliparous women	535	626	0.005 (0.035)	0.891	0.022 (0.044)	0.623
Parous women	5333	6276	0.023 (0.011)	0.029	0.029 (0.014)	0.040
Parous women + age at 1st birth adjustment	5333	6276	0.021 (0.011)	0.051	0.026 (0.014)	0.064
Parous women + age at menopause adjustment	5333	6276	0.023 (0.011)	0.030	0.029 (0.014)	0.041
Parous women + ages at 1st birth + age at menopause adjustments	5333	6276	0.021 (0.011)	0.049	0.026 (0.014)	0.063
Nulliparous women—Model D^e
All	1068	1238	− 0.016 (0.025)	0.517	0.013 (0.033)	0.696
Parous women—Model E^c
All	9561	11,283	0.027 (0.008)	0.001	0.033 (0.011)	0.002
All + age at 1st birth adjustment	9561	11,283	0.026 (0.008)	0.001	0.033 (0.011)	0.003

BMI body mass index, ICMD International Consortium of Mammographic Density, SE standard error
^aA multi-level linear regression model was used to estimate the difference in square-root per cent density and dense area associated with each per year increase in age at menarche. Models specified correlations of women (level 1) within population groups (level 2)
^bBMI ≤ 20 category combined with BMI 20.1–25.0 categories due to lack of convergence
^cModels A (all women) and E (parous women): adjusted for age at mammogram³, menopausal status, age at mammogram*menopausal status interaction term, BMI³, use of HRT, mammogram view, calibration, reader, parity, height
^dModels B (pre-menopausal women) and C (post-menopausal women): adjusted for age at mammogram³, BMI³, use of HRT, mammogram view, calibration, reader, parity, height
^eModel D (nulliparous women): adjusted for age at mammogram³, menopausal status, age at mammogram* menopausal status interaction term, BMI³, use of HRT, mammogram view, calibration, reader, height
^fDense area models: BMI² adjusted for instead of BMI³

Table 3. Association of per cent density and dense area with adult height in pooled ICMD studies
	No. of women	No. of measurements	Per cent density^a		Dense area^a,e
	No. of women	No. of measurements	Changes in square-root per cent density (SE)	P value	Changes in square-root dense area (SE)	P value
Height (categorical) (cm)
< 155	2718	3240	0		0
155–159.9	2551	3027	− 0.041 (0.037)	0.259	0.048 (0.048)	0.319
160–164.9	2683	3151	− 0.056 (0.038)	0.141	0.096 (0.050)	0.057
≥ 165	2480	2900	− 0.075 (0.042)	0.076	0.118 (0.055)	0.034
Linear associations with a 10 cm increase in adult height
All women—Model A^b
All, without BMI adjustment	10,432	12,318	0.044 (0.023)	0.054	0.069 (0.028)	0.012
All, without age at menarche adjustment	10,432	12,318	− 0.057 (0.021)	0.007	0.062 (0.028)	0.025
All, with BMI and menarche adjustment	10,432	12,318	− 0.060 (0.021)	0.005	0.059 (0.028)	0.034
By BMI strata: (kg/m²)
≤ 20.0	652	752	0.042 (0.075)	0.569	0.184 (0.073)	0.011
20.1–25.0	3882	4551	− 0.008 (0.034)	0.811	0.112 (0.041)	0.006
25.1–30.0	3328	3937	− 0.043 (0.037)	0.241	0.121 (0.050)	0.015
> 30.0	2570	3078	− 0.138 (0.040)	0.001	− 0.026 (0.060)	0.670
Subset by height measurement method
Measured	7062	8389	− 0.040 (0.026)	0.125	0.084 (0.034)	0.015
Self-reported	3370	3929	− 0.096 (0.036)	0.007	0.020 (0.047)	0.676
Pre-menopausal women—Model B^c
All	4221	5000	− 0.049 (0.032)	0.130	0.074 (0.044)	0.092
Subset by parity
Nulliparous women	478	553	− 0.201 (0.089)	0.024	− 0.012 (0.117)	0.922
Parous women	3743	4447	− 0.023 (0.034)	0.503	0.099 (0.047)	0.034
Parous women + age 1st birth adjustment	3743	4447	− 0.024 (0.034)	0.490	0.099 (0.047)	0.033
Post-menopausal women—Model C^c
All	5747	6769	− 0.067 (0.028)	0.017	0.047 (0.036)	0.201
All + age at menopause adjustment	5747	6769	− 0.070 (0.028)	0.013	0.043 (0.036)	0.239
Subset by parity
Nulliparous women	531	622	− 0.144 (0.081)	0.076	− 0.084 (0.098)	0.389
Parous women	5216	6147	− 0.041 (0.029)	0.163	0.090 (0.039)	0.019
Parous women + age at 1st birth adjustment	5216	6147	− 0.045 (0.029)	0.121	0.085 (0.038)	0.027
Parous women + age at menopause adjustment	5216	6147	− 0.043 (0.029)	0.139	0.087 (0.038)	0.023
Parous women + age at 1st birth + age at menopause adjustments	5216	6147	− 0.047 (0.029)	0.107	0.083 (0.038)	0.031
Nulliparous women—Model D^d
All	1059	1229	− 0.197 (0.060)	0.001	− 0.043 (0.075)	0.563
Parous women—Model E^b
All	9321	11,024	− 0.036 (0.022)	0.111	0.090 (0.030)	0.002
All + age at 1st birth adjustment	9321	11,024	− 0.038 (0.022)	0.090	0.088 (0.030)	0.003

BMI body mass index, ICMD International Consortium of Mammographic Density, SE standard error
^aA multi-level linear regression model was used to estimate the difference in square-root per cent density and dense area associated with each 10 cm increase in height. Models specified correlations of women (level 1) within population groups (level 2)
^bModels A (all women) and E (parous women): adjusted for age at mammogram³, menopausal status, age at mammogram* menopausal status interaction term, BMI³, use of HRT, mammogram view, calibration, reader, parity, age at menarche
^cModels B (pre-menopausal women) and C (post-menopausal women): adjusted for age at mammogram³, BMI³, use of HRT, mammogram view, calibration, reader, parity, age at menarche
^dModel D (nulliparous women): adjusted for age at mammogram³, menopausal status, age at mammogram* menopausal status interaction term, BMI³, use of HRT, mammogram view, calibration, reader, age at menarche
^eDense area models: BMI² adjusted for instead of BMI³

Authors and Disclosures

Sarah V. Ward¹, Anya Burton^2,3, Rulla M. Tamimi⁴, Ana Pereira⁵, Maria Luisa Garmendia⁵, Marina Pollan^6,7, Norman Boyd⁸, Isabel dos-Santos-Silva⁹, Gertraud Maskarinec¹⁰, Beatriz Perez-Gomez^6,7, Celine Vachon¹¹, Hui Miao¹², Martín Lajous¹³, Ruy López-Ridaura¹³, Kimberly Bertrand¹⁴, Ava Kwong^15–17, Giske Ursin^18–20, Eunjung Lee²¹, Huiyan Ma²², Sarah Vinnicombe²³, Sue Moss²⁴, Steve Allen²⁵, Rose Ndumia²⁶, Sudhir Vinayak²⁶, Soo-Hwang Teo^27,28, Shivaani Mariapun²⁸, Beata Peplonska²⁹, Agnieszka Bukowska-Damska³⁰, Chisato Nagata³¹, John Hopper³², Graham Giles^32,33, Vahit Ozmen³⁴, Mustafa Erkin Aribal³⁵, Joachim Schüz¹, Carla H. Van Gils³⁶, Johanna O. P. Wanders³⁶, Reza Sirous³⁷, Mehri Sirous³⁸, John Hipwell³⁹, Jisun Kim⁴⁰, Jong Won Lee⁴⁰, Caroline Dickens⁴¹, Mikael Hartman^12,42, Kee-Seng Chia⁴³, Christopher Scott¹¹, Anna M. Chiarelli⁴⁴, Linda Linton⁸, Anath Arzee Flugelman⁴⁵, Dorria Salem⁴⁶, Rasha Kamal⁴⁶, Valerie McCormack²* and Jennifer Stone¹

¹School of Population and Global Health, The University of Western Australia, Perth, Australia. ²Environment and Lifestyle Epidemiology Branch, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France. ³Translation Health Sciences, University of Bristol, Bristol, UK. ⁴Population Health Sciences, Weill Cornell Medical College, Cornell University, New York, USA. ⁵Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile. ⁶Cancer and Environmental Epidemiology Unit, Instituto de Salud Carlos III, Madrid, Spain. ⁷Consortium for Biomedical Research in Epidemiology and Public Health (CIBERESP), Madrid, Spain. ⁸Princess Margaret Cancer Centre, Toronto, ON, Canada. ⁹Department of Non-Communicable Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, UK. ¹⁰University of Hawaii Cancer Center, Honolulu, HI, USA. ¹¹Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA. ¹²Saw Swee Hock School of Public Health, National University of Singapore, Singapore City, Singapore. ¹³Instituto Nacional de Salud Pública, Cuernavaca, Mexico. ¹⁴Slone Epidemiology Center at, Boston University, Boston, MA, USA. ¹⁵Division of Breast Surgery, Faculty of Medicine, University of Hong Kong, Pok Fu Lam, Hong Kong, China. ¹⁶Department of Surgery and Cancer Genetics Center, Hong Kong Sanatorium and Hospital, Pok Fu Lam, Hong Kong, China. ¹⁷Hong Kong Hereditary Breast Cancer Family Registry, Pok Fu Lam, Hong Kong, China. ¹⁸Cancer Registry of Norway, Oslo, Norway. ¹⁹Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway. ²⁰Department of Preventive Medicine, University of Southern California, Los Angeles, CA, USA. ²¹Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, USA. ²²Department of Population Sciences, City of Hope National Medical Center, Duarte, CA, USA. ²³Division of Cancer Research, Ninewells Hospital and Medical School, University of Dundee, Dundee, Scotland, UK. ²⁴Wolfson Institute of Preventive Medicine, Queen Mary University of London, London, UK. ²⁵Department of Imaging, Royal Marsden NHS Foundation Trust, London, UK. ²⁶Aga Khan University Hospital, Nairobi, Kenya. ²⁷Breast Cancer Research Group, University Malaya Medical Centre, University Malaya, Kuala Lumpur, Malaysia. ²⁸Cancer Research Malaysia, Subang Jaya, Malaysia. ²⁹Department of Environmental Epidemiology, Nofer Institute of Occupational Medicine, Łodź, Poland. ³⁰Department of Physiology, Pathophysiology and Clinical Immunology,, Medical University of Lodz., Łodź, Poland. ³¹Department of Epidemiology and Preventive Medicine, Graduate School of Medicine, Gifu University, Gifu, Japan. ³²Centre for Epidemiology and Biostatistics, Melbourne School of Population and Global Health, University of Melbourne, Melbourne, VIC, Australia. ³³Cancer Epidemiology Centre, Cancer Council Victoria, Melbourne, VIC, Australia. ³⁴Faculty of Medicine, Istanbul University, Istanbul, Turkey. ³⁵Department of Radiology, School of Medicine, Marmara University, Istanbul, Turkey. ³⁶Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands. ³⁷Radiology Department, George Washington University Hospital, Washington, DC, USA. ³⁸Radiology Department, Isfahan University of Medical Sciences, Isfahan, Iran. ³⁹Centre for Medical Image Computing, University College London, London, UK. ⁴⁰Asan Medical Center, Seoul, Republic of Korea. ⁴¹Department of Internal Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. ⁴²Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore City, Singapore. ⁴³NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore. ⁴⁴Ontario Breast Screening Program, Cancer Care Ontario, Toronto, ON, Canada. ⁴⁵National Cancer Control Center, Lady Davis Carmel Medical Center, Faculty of Medicine, Technion-Israel Institute Technology, Haifa, Israel. ⁴⁶Woman Imaging Unit, Radiodiagnosis Department, Kasr El Aini, Cairo University Hospitals, Cairo, Egypt.

*Correspondence
McCormackV@iarc.fr

Author Contributions
The study was conceptualized and designed by VM, JSt and ABur and supervised by VM and JSt. Data were curated by GM, BPG, CV, HMi, ML, RLR, AP, MLG, RMT, KB, AK, GU, EL, HMa, SVinn, SMo, SA, RN, SVina, SHT, SMa, BP, AB-D, CN, JSt, JHo, GG, VO, MEA, JSc, CVG, JOPW, RS, MS, JHi, JK, JWL, CD, MH, KSC, CS, AMC, LL, MP, AAF, DS, RK, NB, IdSS and VM. Centralized breast density readings were performed by VM, IdSS and NB, and data harmonization was conducted by ABur. Primary data analyses were performed by SW, with additional analyses and input from VM and ABur. Results were interpreted by SW, JSt, VM, ABur, GM, AP, MLG, KB, BP, MP, NB and IdSS. The manuscript was drafted by SW, JSt, VM and ABur and revised by SW and JSt. All authors reviewed the final manuscript. All authors read and approved the final manuscript.

Competing interests
ML declares a non-restricted investigator-initiated grant from AstraZeneca and minor support from Swiss Re.