This review evidences the potential of dPCR as a useful tool that could contribute to improve the diagnosis and clinical management of infection in critical care medicine. Although most of the published works consist of pilot/exploratory studies, they show the potential of dPCR, supporting the development of further, larger studies aimed to validate the use of this technology in this field.
AMR: Antimicrobial resistance genes; AUROC: Area under the receiver operating curve; BSIs: Bloodstream infections; CD40LG: CD40 Ligand; cfDNA: Cell-free deoxyribonucleic acid; COVID-19: Coronavirus disease 2019; ddPCR: Droplet digital polymerase chain reaction; DNA: Deoxyribonucleic acid; dPCR: Digital polymerase chain reaction; EBV: Epstein-Barr Virus; HLA-DRA: Major histocompatibility complex class II, DR alpha molecule; ICOS: Inducible T Cell Costimulator; ICU: Intensive care unit; IgG: Immunoglobulin G; IgM: Immunoglobulin M; LCN2: Lipocalin 2; MDR: Multidrug-resistance; miRNA: Micro ribonucleic acid; MMP8: Matrix metalloproteinase-8; mNGS: Metagenomic Next-Generation Sequencing (); MTB: Mycobacterium tuberculosis; mtDNA: Mitochondrial DNA; PCR: Polymerase chain reaction; PCT: Procalcitonin; qPCR: Real-time polymerase chain reaction; rDNA: Ribosomal deoxyribonucleic acid; RNA: Ribonucleic acid; SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2; sCAP: Severe community acquired pneumonia; SIRS: Systemic inflammatory response syndrome; VAP: Ventilator-associated pneumonia.
This work was funded by Instituto de Salud Carlos III (ISCIII), (Project Code PI19/00590), co-funded by European Regional Development Fund (ERDF) · European Social Fund (ESF), "A way to make Europe"/"Investing in your future") (JFBM), and also by a Research Grant 2020 from ESCMID (APT). APT, IM and AF salaries were funded by the Sara Borrell Research Grant (CD18/00123), Rio Hortega (CM18/00157) and PFIS (FI20/00278) Research Grants, respectively, from ISCIII and co-funded by ERDF/ESF, "A way to make Europe"/"Investing in your future").
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Crit Care. 2022;26(63) © 2022 BioMed Central, Ltd.
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