Parasitic Disease Surveillance, Mississippi, USA

Table 1. Results of microscopic examination and real-time PCR testing for soil-transmitted helminth and Strongyloides stercoralis infection on postdiagnostic fecal samples from patients at University of Mississippi Medical Center, Jackson, Mississippi, USA*
Method	Inhibition and extraction control	S. stercoralis	Necator americanus	Ascaris lumbricoides	Trichuris trichiura	Ancylostoma spp.	Other parasite species
Saturated salt centrifugal flotation	NA	0/507 (0)	0/507 (0)	0/507 (0)	0/507 (0)	0/507 (0)	0/507 (0)
Real-time PCR	594/631 (94.1)	1/594 (0.2)	0/594 (0)	0/224 (0)	0/224 (0)	0/224 (0)	NA

*Values are no. (%) unless indicated. NA, not applicable.

Table 2. Results of multiplex serologic testing for antibodies suggesting prior exposure to *Toxocara* spp., *Giardia duodenalis*, and *Cryptosporidium* spp. on 1,960 postdiagnostic serum samples from patients at University of Mississippi Medical Center, Jackson, Mississippi, USA*
Parasite antigen used
Toxocara spp. Tc-CTL-1	S. stercoralis rSs-NIE-1 plus CrAg-ELISA†	G. duodenalis VSP3	C. parvum Cp17	C. parvum Cp23	C. parvum Cp17 + Cp23‡	C. parvum Cp17 + Cp23 and G. duodenalis VSP3
172 (8.8)	4 (0.2)	111 (5.7)	646 (33.0)	1,076 (54.9)	538 (27.4)	38 (1.9)

*All values are no. (%).
†8 samples were found to be positive by the rSs-NIE-1 MAGPIX multiplex serologic assay (ThermoFisher, https://www.thermofisher.com), but only 4 reacted in the confirmatory S. stercoralis crude L3 larval antigen (CrAg) ELISA.
‡Only samples reactive to both Cp17 and Cp23 were considered positive for Cryptosporidium spp. exposure.

Authors and Disclosures

Richard S. Bradbury¹, Meredith Lane, Irene Arguello, Sukwan Handali, Gretchen Cooley, Nils Pilotte, John M. Williams, Sam Jameson, Susan P. Montgomery, Kathryn Hellmann, Michelle Tharp, Lisa Haynie, Regina Galloway, Bruce Brackin, Brian Kirmse, Lisa Stempak, Paul Byers, Steven Williams, Fazlay Faruque and Charlotte V. Hobbs¹

Centers for Disease Control and Prevention, Atlanta, Georgia, USA (R.S. Bradbury, M. Lane, S. Handali, G. Cooley, S.P. Montgomery); Synergy America, Inc., Atlanta (M. Lane); University of Mississippi Medical Center, Jackson, Mississippi, USA (I. Arguello, J.M. Williams, S. Jameson, K. Hellmann, M. Tharp, L. Haynie, R. Galloway, B. Kirmse, L. Stempak, F. Faruque, C.V. Hobbs); Smith College, North Hampton, Massachusetts, USA (N. Pilotte, S. Williams); Mississippi State Department of Health, Jackson (B. Brackin, P. Byers)

Address for correspondence
Richard S. Bradbury, School of Health and Life Sciences, Federation University, Berwick, 3806, VIC, Australia; email: r.bradbury@federation.edu.au; Charlotte V. Hobbs, Department of Pediatrics, Division of Infectious Disease, Department of Microbiology, University of Mississippi Medical Center, Batson Children's Hospital, 2500 N State St, Jackson, MS 39216, USA; email: chobbs@umc.edu

¹These authors contributed equally to this article.

About the Author
Dr. Bradbury is a senior lecturer in microbiology and molecular biology at Federation University in Berwick, Victoria, Australia, and is a microbiologist with expertise in laboratory diagnostics and parasitic diseases. His research interests include strongyloidiasis, soil-transmitted helminths, zoonoses, and emerging parasitic diseases. Dr. Hobbs is a professor of pediatric infectious disease and attending physician at Children's of Mississippi, University of Mississippi Medical Center, Jackson, Mississippi, USA. Her research interests include parasitic diseases in children in resource-limited settings.

Parasitic Disease Surveillance, Mississippi, USA

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