Blood Cell Count and the Presence or Absence of Infection in Venous Ulcers Treated With Platelet-Rich Plasma

Beatriz Guitton Renaud Baptista de Oliveira, DN, RN; Joyce Beatriz de Abreu Castro, MSN, RN; Bruna Maiara Ferreira Barreto Pires, RN, PhD; Márcia de Assunção Ferreira, DN, RN; Jane Marcy Neffá Pinto, PhD, MD; Lenise Arneiro Teixeira, DSC, BF


Wounds. 2021;33(5):113-118. 

In This Article


Of the 17 included patients, 10 (59%) were male. With regard to age, 10 patients (59%) were aged 60 years to 80 years, followed by 6 patients (35.4%) aged 50 years to 59 years and 1 patient (5.9%) between 40 years and 49 years. Only 3 patients (18%) reported alcohol misuse, and 4 patients (23.5%) reported being smokers. Wounds were predominantly located in the malleolar region (72.2%), followed by the dorsum of the foot (27.7%). For all patients, current lesion onset had started more than 5 years ago. The average area of ulcers was 29.6 ± 23.9 cm2.

At baseline, 10 patients (58.8%) had wound infection. Wound infection was detected in 3 patients (17.6%) and 1 patient (5.9%)during week 6 and week 12 of treatment, respectively. Complete wound healing was achieved after 12 weeks of treatment in 3 patients (18%).

The McNemar test and χ 2 test revealed significant differences in the presence of infection between baseline and the sixth week of treatment (P = .0039) and between baseline and the 12th week of treatment (P = .0078).

Three microbial collections were performed (on week 1, week 6, and week 12) on the 17 included patients, yielding 48 total samples; 5 patients' ulcers healed before completing the 90 days of treatment and thus were not swabbed after complete closure of wound. Of the total samples, 22 were positive for P aeruginosa. Of the P aeruginosa samples, 6 were positive from the first collection, 8 from the second collection, and 8 from the third collection. Regarding S aureus, only 6 samples (10%) were positive for this microorganism. For patients with S aureus, 2 samples were positive for this bacterium from the first, second, and third collections.

In the context of leukocyte cells, no differences were observed between the numbers of different leukocyte cells detected in WBC counts before and after treatment. White blood cell counts produced normal values for most of the patients, as indicated in Table 2.

Only 1 (6%) of the 2 (12%) patients who had elevated leukocytes before treatment endured wound infection; for this patient, infection was no longer present by week 6 of treatment, and leukocyte counts normalized. For the other patient, leukocytes remained above the reference range, even after treatment when the wound had already healed (Table 2).