Mean Platelet Volume, Platelet Distribution Width, and Platelet Count, in Connection With Immune Thrombocytopenic Purpura and Essential Thrombocytopenia

Eunyup Lee, MD; Miyoung Kim, MD; Kibum Jeon, MD; Jiwon Lee, MD; Jee-Soo Lee, MD; Han-Sung Kim, MD; Hee Jung Kang, MD; Young Kyung Lee, MD


Lab Med. 2019;50(3):279-285. 

In This Article

Materials and Methods


A total of 153 individuals whose complete blood count (CBC) results were within the reference ranges and who did not have any underlying diseases were enrolled in the control group. The hyperdestructive-thrombocytopenia group comprised 35 patients with ITP, and the hyperproductive-thrombocytosis group comprised 48 patients with ET; none of the patients in either group had previously received treatment. ET was diagnosed based on bone-marrow examination and molecular study of JAK2and/or CALR mutation status, as well as clinical observations and other laboratory data according to the World Health Organization (WHO) classification.[13]

The specimens were collected randomly at Hallym Sacred Heart Hospital, Anyang, South Korea, between January 2009 and April 2016. This study was approved by the Institutional Review Board/Ethics Committee of Hallym University Sacred Heart Hospital (IRB no. 2016-I030).

CBCs and Measurement of MPV and PDW

In the control group, CBCs (including simultaneous measurements of MPV and PDW) were obtained using the ADVIA2120i (Siemens AG) within 1 hour of specimen collection according to manufacturer instructions. Hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW), white blood cell (WBC) count, PLT count, PDW, and MPV results were included in the analyses.


The Shapiro-Wilk test was used to test for normality. Values are expressed as mean (SD) for normally distributed data and as medians (interquartile ranges) for non–normally distributed data. We used Kruskal-Wallis and Mann-Whitney analyses to compare CBC parameters (Hb, MCV, mean corpuscular hemoglobin [MCH], MCHC, PDW, WBC, PLT count, and MPV) among the 3 groups and their subgroups. Spearman rank correlation analysis was used to determine associations between parameters. We performed all statistical analyses using SPSS software, version 22.0 (IBM Corporation). P values less than .05 were considered statistically significant.