Detection of Herpes Simplex Virus and Varicella-Zoster Virus by Traditional and Multiplex Molecular Methods

Dejan Nikolic, MD, PhD; Debra Kohn; Belinda Yen-Lieberman, PhD; Gary W Procop, MD, MS

Disclosures

Am J Clin Pathol. 2018;151(1):122-126. 

In This Article

Results

Thirty-eight (40%) of the 95 specimens assessed for HSV contained either HSV-1 or HSV-2, as determined by the consensus criteria described above; 19 (20%) specimens contained HSV-1 and 19 (20%) contained HSV-2. One specimen gave an "Invalid" test result for the Solana assay and was excluded from the further assessment of that assay. Forty-three (45%) of the 93 specimens assessed for VZV were shown to contain the virus, as determined by the consensus criteria described above. Three of these specimens had an insufficient cellularity for a DFA assessment. Interestingly, one of the three with insufficient cellularity tested positive for VZV by both molecular assays. None of the specimens contained both HSV and VZV (ie, no dual infections were detected).

The true-positive, true-negative, false-positive, and false-negative categorizations are shown in the Table 1 . Significantly more true-positive HSV-containing specimens were detected by the Solana HSV1 + 2/VZV Assay (P = .006) and the ARIES HSV 1&2/VZV Combination Assay (P = .001) compared with the HSV ELVIS Test System. Similarly, significantly more true-positive VZV-containing specimens were detected by the Solana HSV1 + 2/VZV Assay (P = .003) and the ARIES HSV 1&2/VZV Combination Assay (P < .001) compared with the VZV DFA. There was no significant difference between the molecular assays for the detection of HSV (P = .56) or VZV (P = .15).

The sensitivity/specificity of the HSV ELVIS Test System and VZV DFA were 71.1%/93.2% and 71.4%/100%, respectively. The sensitivity/specificity of the ARIES HSV 1&2/VZV Combination Assay for the detection of HSV were 94.9%/93.2%, whereas for VZV these were 100%/96.0%, respectively. The sensitivity/specificity of the Solana HSV1 + 2/VZV Assay for the detection of HSV were 94.7%/100%, whereas for VZV, these were 95.3%/100%, respectively. After medical record review, it was determined that HSV and VZV were detected but clinically unsuspected in 5.4% (5/93) and 4.2% (4/95) of the specimens, respectively.

Comments

3090D553-9492-4563-8681-AD288FA52ACE

processing....