Collection of Nasal Secretions and Tears and Their Use in Allergology

Sveva Castelli; Stefania Arasi; Ruby Pawankar; Paolo M. Matricardi


Curr Opin Allergy Clin Immunol. 2018;18(1):1-9. 

In This Article

Detection of Immunoglobulin E in Situ: Nasal Test

To overcome the main problems of nasal secretions sampling, some authors have developed an alternative method in which the test device is put in direct contact with the nasal mucosa, the 'Nasal Test'.[70] In this procedure, the solid phase coupled with allergens is placed directly on nasal mucosa behind the internal ostium and kept in place for 5 min.[70] Allergen-IgE antibody incubation is so performed in situ, in the same conditions in which IgE-mediated reaction takes place.[70] Compared to the 3 h incubation time needed in collected nasal secretions to reach the maximum value, the Nasal Test is described as a time saving procedure in which the maximum value is reached within 5 min incubation.[70] Good sensitivity, specificity, and reproducibility of the method are reported.[70,71] The Nasal Test seems to be suitable and well accepted even by children.[70,71] However, there are also limitations to be considered: only few studies has been performed with this method.[70,71] Furthermore, the allergens come into contact with the mucosa of the patient. The Nasal Test is therefore at the same time a provocation test and might provoke a reaction. The safety of the procedure has thus to be carefully investigated.

An overview of the principal advantages and disadvantages of each method for nasal secretions collection is shown in Table 2.