Zika in Newborns: A Q&A for Clinicians

Laurie Scudder, DNP, PNP

Disclosures

February 03, 2016

Evaluation of Newborns with Zika Exposure

Which newborns should be tested for Zika virus infection?

Testing for Zika virus infection is recommended for infants born to women who traveled to or resided in an area with ongoing Zika virus transmission during pregnancy who were 1) diagnosed with microcephaly or intracranial calcifications detected prenatally or at birth, or 2) who have mothers with positive or inconclusive test results for Zika virus infection.

How are infants diagnosed with Zika virus infection?

Zika virus infection can be diagnosed by performing reverse transcriptase-polymerase chain reaction (RT-PCR) on infant serum. Serology assays can also be used to detect Zika virus-specific IgM and neutralizing antibodies. However, since it has not been established which test is most reliable for a diagnosis in infants, RT-PCR and IgM tests should both be performed. Plaque-reduction neutralization testing (PRNT) can also be performed to measure virus-specific neutralizing antibodies and differentiate from other flaviviruses.

If Zika virus testing of a newborn is indicated, how is the test ordered?

There are no commercially available tests for Zika virus. Zika virus testing is performed at the CDC Arbovirus Diagnostic Laboratory and at some state and territorial health departments. Healthcare providers should contact their state and local health department to facilitate testing. See the Diagnostic Testing webpage for information on how to obtain Zika testing.

If Zika virus testing of a newborn is indicated, what specimens are recommended?

Zika virus RT-PCR and serology assays can be performed on infant serum or serum or plasma collected from the umbilical cord. If cerebrospinal fluid (CSF) specimens are available, Zika virus RT-PCR should be performed; however, CSF specimens should not be collected for the sole purpose of Zika virus testing. Other specimens that can be tested include the placenta and the umbilical cord. Histopathologic examination and immunohistochemical staining can be performed. Zika virus RT-PCR on fixed and frozen tissue should also be considered.

Should healthcare providers report infants with positive or inconclusive Zika virus test results?

Healthcare providers should report positive or inconclusive results to their state or territorial health department. As an arboviral disease, Zika virus disease is a nationally notifiable condition.

What should healthcare providers do to evaluate infants with positive or inconclusive Zika virus test results?

A thorough physical examination should be performed, including careful measurement of the head circumference, length, weight, and assessment of gestational age. Cranial ultrasound is recommended unless it was performed as part of prenatal screening in the third trimester and clearly showed no abnormalities of the brain. Ophthalmologic evaluation is recommended as well as repeat hearing screen at six months of age. Continued evaluation of developmental characteristics and milestones, including head circumference, is recommended through the first year of life.

If a mother had Zika virus infection during pregnancy but the newborn tests negative for Zika virus, what is recommended for additional follow-up?

If the newborn does not have abnormal findings on examination, the infant should receive routine pediatric care including measurement of growth and development, and appropriate evaluation and follow-up for any clinical findings that arise. If the newborn has abnormal findings on examination, diagnostic testing for other causes of the newborn's conditions should be performed including testing for other congenital viral infections if indicated.

If a mother had Zika virus infection during pregnancy, should she breastfeed her infant?

Although Zika virus RNA has been detected in breast milk, transmission of Zika infection through breastfeeding has not been documented. Based on available evidence, the benefits of breastfeeding infants outweigh any theoretical risk related to Zika virus infection.

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