Proteomics Strategies to Analyze HPV-Transformed Cells: Relevance to Cervical Cancer

Fabio Di Domenico; Federico De Marco; Marzia Perluigi

Disclosures

Expert Rev Proteomics. 2013;10(5):461-472. 

In This Article

Five-Year View

Proteomics strategies have continuously evolved to better describe the molecular events occurring in disease processes and to search for biomarkers of disease. In the case of HPV infection, proteomics studies underlie a 'regulatory role' of HPV oncogenes, where specific interactions between the E6 and E7 viral gene products and cellular proteins occur thus affecting cellular growth, transformation and immortalization. Understanding these interactions have been the focus of major research and advancement of proteomics platforms contributed to identify which intracellular target are mainly involved in tumor development. However, regulation of protein function should consider the fact that proteins undergo numerous PTM – phosphorylations, glycosylations, acetylation – in addition to other types of irreversible modifications which are critical to their functions, as they can affect activity, stability, localization and turnover. Thus, next generation studies should employ selective proteomics approaches which aim to characterize specific PTM more than simply looking at difference in protein expression levels. By following this approach, novel putative marker may emerge and may also contribute to better understand the mechanism of disease pathogenesis and progression. To this purpose, the field of proteomics will continuously advance by improving the accuracy, sensitivity and speed of MS and also by expansion of bioinformatics tools.

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