Novel Type of Protein Chip for Multiplex Detection of Autoantibodies

Christer Wingren


Expert Rev Proteomics. 2013;10(5):417-420. 

In This Article

Significance of the Results

Today, the number of functional as well as analytical protein microarray platforms which are based on protein probes, microarray adapted by molecular design, are still very few. The route adopted by the authors is very interesting, providing the arrayed proteins with in-built means for both immobilization and detection (of the amount of deposited proteins). These properties could be explored in several protein microarray layouts, certainly not limited to functional protein microarrays for screening of autoantibodies only. Furthermore, the array set-up also allowed on-chip denaturation of the arrayed proteins, which could prove essential, enabling both denatured and non-denatured antigens to be explored in the screening of autoantibodies.

The use of conventional functional protein microarrays for screening of serum autoantibodies as potential disease biomarkers have been explored in a number of recent studies targeting a variety of diseases. Still, the need for additional, high-performing functional protein microarray set-ups is significant. More specifically, the development of novel platforms, enabling the arrayed protein probes to be displayed in (more) suitable formats, for example, denatured, to be recognized and bound by autoantibodies will be essential. The authors demonstrated that hepatocellular carcinoma-related IgG autoantibodies against denatured antigens could be detected in the sera of hepatitis C virus-positive patients, thus expanding the repertoire of potential biomarkers. Taken together, the use of functional protein microarrays for multiplex detection and screening of disease-associated autoantibodies will continue to be high and most likely even expand in the future.