HAMLET: Functional Properties and Therapeutic Potential

James Ho CS; Anna Rydström; Maria Trulsson; Johannes Bålfors; Petter Storm; Manoj Puthia; Aftab Nadeem; Catharina Svanborg


Future Oncol. 2012;8(10):1301-1313. 

In This Article

Cell Death in Response to HAMLET-like Complexes

BAMLET has been the focus of several interesting studies addressing the interaction with tumor cells.[55,66] BAMLET reproduces many aspects of the tumoricidal response to HAMLET including the morphologic changes, internalization and loss of viability. Rammer et al. have shown that BAMLET activates caspase-dependent apoptosis-like cell death and autophagy, but does not kill cells by these mechanisms. In addition, the BAMLET complex has been shown to activate the lysosomal cell death pathway.[55] The ELOA complexes trigger cell death, accompanied with combined staining of acridine orange and ethidium bromide, following the accumulation of ELOA in the vicinity of the cell membrane.[67] Several other complexes derived from β-lactoglobulin and parvalbumin have also been shown to trigger cytotoxic responses involving membrane depolarization and membrane damage.[43]