HAMLET: Functional Properties and Therapeutic Potential

James Ho CS; Anna Rydström; Maria Trulsson; Johannes Bålfors; Petter Storm; Manoj Puthia; Aftab Nadeem; Catharina Svanborg


Future Oncol. 2012;8(10):1301-1313. 

In This Article

HAMLET Treatment Delays Bladder Cancer Development in a Mouse Model

The human bladder cancer study was not designed as a controlled, therapeutic study. To evaluate whether HAMLET is efficient as a topical therapeutic agent in bladder cancer, the authors therefore used the mouse MB49 bladder carcinoma model.[75] Rapidly growing tumors were established by intravesical inoculation of tumor cells and HAMLET was applied topically. Using tumor size as the therapeutic end point, a significant therapeutic effect of HAMLET was detected. A reduction in tumor development was observed in HAMLET-treated mice compared with the control group. Furthermore, by in vivo imaging of Alexa–Fluor-labeled HAMLET, accumulation was detected in tumor tissue for at least 24 h, but not in the healthy tissues surrounding the tumor. The results show that HAMLET is active as a tumoricidal agent with selective uptake in bladder tumor tissue and suggest that topical HAMLET administration should be further tested in patients with bladder cancer.