Chikungunya Outbreak

Cambodia, February-March 2012

Sowath Ly, PhD; Sopheak Sorn, MA; Arnaud Tarantola, MD; Lydie Canier, MSc; Philippe Buchy, PhD; Veasna Duong, PhD; Ilin Chuang, MD; Steven Newell, PhD; Sovann Ly, MD; Touch Sok, MD; Vandy Som, MD; Meng Chuor Char, MD; Chantha Ngan, MD; Leakhann Som, MD; Maria Concepcion Roces, MD

Disclosures

Morbidity and Mortality Weekly Report. 2012;61(37):737-740. 

In This Article

Abstract and Introduction

Introduction

Chikungunya virus (CHIKV) is an alphavirus transmitted to humans through the bite of infected Aedes mosquitoes.[1] CHIKV causes fever and usually is not fatal, but can cause debilitating joint pains or, in rare instances, severe illness. The East/Central/South African strain of chikungunya has been emerging in Asia since 2006, first in the Indian subcontinent, then Thailand. This report describes the characteristics of a local outbreak linked with chikungunya reemergence in a rural Asian setting. Sporadic cases of chikungunya were identified in Cambodia in 2011.[2] Antibodies to CHIKV have been detected in serum collected in Cambodia in 2007, but the strain could not be identified for those cases (U.S. Naval Medical Research Unit 2, unpublished data, 2012). On March 7, 2012, several cases of rash with fever were reported among village residents of Trapeang Roka in Kampong Speu Province, Cambodia. Subsequent field investigation revealed that four of six blood samples from affected persons were positive for CHIKV by polymerase chain reaction (PCR) at U.S. Naval Medical Research Unit 2 in Phnom Penh. Investigators from the Cambodian Communicable Disease Control Department, National Malaria Center, Institut Pasteur du Cambodge (IPC), local health centers, and village authorities conducted a seroprevalence study of village residents on March 26 to gather information for response planning and control efforts. The outbreak affected families throughout the village, and 44.7% of the population tested had evidence of infection by CHIKV, which affected all age groups. Public health agencies and policymakers in affected and nearby unaffected areas of Asia and elsewhere should be alert to the potential spread and reemergence of CHIKV.

Trapeang Roka has a population of approximately 695 persons in 134 houses; most adults are farmers or factory workers. For the survey, the village was divided into six sectors created by the roads crossing through it. One team of investigators was assigned to each sector, and a central blood-sampling station was established. Teams worked outward from the center of the village, going from house-to-house. In each house, all occupants present were interviewed. Those who were in the field or factory rather than at home were interviewed when they returned to the village in the evening. Informed consent was obtained in the Khmer language from all adult residents and parents or guardians of children; none refused to participate. A standardized questionnaire was used to gather information on demographics and recent (since the February 14–15 rains) or current self-reported symptoms including joint pains, fever >38°C (axillary or subjective), and rash. A clinical case was defined as one or more of these symptoms in a person from this village with onset after February 14 through March 26, 2012. Blood specimens were obtained for serologic testing; dried blood spots were collected from all persons surveyed and venous samples were collected from febrile patients. Confirmed cases had a positive laboratory test.

At IPC's Virology Unit, immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) testing was performed to detect anti-CHIKV IgM.[3] Serologic testing also was performed by MAC-ELISA for recent infection by dengue (DENV) and Japanese encephalitis B (JEV) viruses.[4] Positive serology for DENV and JEV pointed to recent flavivirus infection, which might present similar symptoms. Patients who were febrile at investigation also were tested by real-time reverse transcriptase PCR for CHIKV and also DENV and JEV. The 91 persons with recent or current symptoms who were negative for CHIKV IgM were screened for malaria by PCR.

The survey included 98 (73.1%) households distributed throughout the village. The team interviewed 425 persons (61.1% of village population; male:female ratio = 0.82); mean age was 26.4 years (range: 1 month–87 years; interquartile range: 10–39 years). Among the 425 persons interviewed were 96 (22.6%) farmers, 89 (20.9%) students, 91 (21.4%) factory workers, and 73 (17.2%) persons who stayed at home; the remaining 76 persons were preschool-aged children, construction workers, vendors, or pig farmers.

Of the 425 persons interviewed, 312 (73.4%) reported that they had at least one of the three defining symptoms since the rains of February 14–15, 2012 (Table). Among those 312 persons, 173 (55.4%) took leave from school or work. One death during that period was identified retrospectively in a woman aged 33 years with no known underlying disease. The woman had fever and intense joint pain, developed neurologic signs, and died within 2 hours of hospital admission. No samples were available for testing. Her two children were symptomatic, CHIKV IgM-positive, and seronegative for dengue and JEV.

MAC-ELISA tests identified CHIKV IgM in 188 (44.2%) of the 425 persons. Of the six febrile persons, venous blood samples could be analyzed from four: two had positive PCR results for CHIKV, bringing the total that were laboratory confirmed to 190 (44.7%) CHIKV-positives (Table). One person with fever and joint pains was positive for DENV-4. PCR results found traces of Plasmodium spp. nucleic acid indicative of incubating or recent malaria in one afebrile person. Of the 190 CHIKV-positive persons identified, 10 (5.3%) reported having none of the three defining symptoms.

The onset of the epidemic was protracted. Among 140 persons with self-reported fever who recalled an onset date and who had positive results for CHIKV (IgM or PCR), most reported onset occurring approximately 3–5 weeks after a 2-day period of rain (Figure 1). The epidemic curve suggested that the CHIKV outbreak began 3 weeks after the rains, lasted about 3 weeks, and was on the verge of ending when this serosurvey was conducted on March 26, 2012.

Figure 1.

Onset of fever among village residents who recalled an onset date and had laboratory confirmed cases of chikungunya virus (CHIKV) infection* — Trapeang Roka, Cambodia, February and March 2012
* Among these 140 persons with self-reported fever who recalled an onset date and who had positive laboratory results for CHIKV, most reported onset occurring approximately 3–5 weeks after a 2-day period of rain.

Laboratory results were analyzed by age group (Figure 2). The analysis revealed a 40% IgM seroprevalence among persons aged ≤5 years; for persons aged 6–45 years, IgM seroprevalence was approximately 50%, declining sharply for each age group after that. Circulation of at least two other viruses was identified, including 11 cases of DENV, seven cases of JEV, and 15 cases of undistinguishable flaviviruses (among these, 10, four, and eight cases, respectively, occurred among CHIKV-positive cases).

Figure 2.

Prevalence of chikungunya immunoglobulin M (IgM) or polymerase chain reaction (PCR) positivity, by age group — Trapeang Roka, Cambodia, March 2012

During a small-scale entomologic assessment conducted during March 29–30, 2012, 123 mosquitoes were collected in Trapeang Roka and 651 mosquitoes were collected from a wider area, including two nearby villages that also reported cases, based on syndromic data. Aedes aegypti comprised 41.4% and 53% of mosquitoes collected in the two areas, respectively, and Culex species comprised 21.1% and 13.8%, respectively; the rest were Anopheles species, and no Aedes albopictus were identified.

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