Effects of Three Types of Honey on Cutaneous Wound Healing

Hatice Ozlem Nisbet, DVM, PhD; Cevat Nisbet, DVM, PhD; Murat Yarim, DVM, PhD; Ahmet Guler, PhD; Ahmet Ozak, DVM, PhD

Disclosures

Wounds 

In This Article

Evaluation Parameters

Planimetry

There was no significant difference between the honey-treated groups and the control group on days 7 and 14. The unhealed area was the highest (P < 0.05) in the control and rhododendron groups on day 21 (Figure 10).

Figure 10.

Planimetry findings of unhealed areas (cm2) on days 7, 14, and 21 of wounds treated with honeys of different botanical origin and untreated control wounds (mean ± SD; n = 32 for each group).
*†Significant differences (P < 0.05)

Histopathological Evaluation

Histopathological examination results are summarized in Table 1. Although the collagen level was significantly higher in the honeytreated groups on days 14 and 21 (P < 0.05), the level was not significantly different between the honey-treated groups and the control group on day 7. On day 7, epithelization significantly increased in the honey-treated groups (P < 0.05) and a tendency to increase was observed later on. On day 21, the wounds of all treatment groups were almost fully epithelialized. A significant increase was noted in the honey-treated groups (P < 0.05) on days 7 and 21, whereas, no difference was observed regarding angiogenesis on day 14. When the macrophage counts were evaluated, although there were significant difference between the control and the honey-treated groups on day 7, the macrophage counts were significantly higher only in the PRH group on day 14 (P < 0.05).

Fibroplasia was significantly higher in the honey-treated groups (P < 0.05) on day 7, and a tendency for fibroplasia to decrease was observed later on. There was no significant difference that could be noted between the treatment groups during the rest of the study.

Biochemical Evaluation

Hydroxyproline levels of all groups are presented in Figure 11. Hydroxyproline levels were higher in the honey-treated groups than in the control group on days 7 and 14. Highest hydroxyproline levels were observed in the PCH group (P < 0.05) on day 14.

Figure 11.

The biochemical findings among groups. Hydroxyproline levels of open wounds in rabbits treated with honeys of different botanical origin and untreated control wounds (mean ± SD; n = 32 for each group).
*†Significant differences (P < 0.05)

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