Effects of Three Types of Honey on Cutaneous Wound Healing

Hatice Ozlem Nisbet, DVM, PhD; Cevat Nisbet, DVM, PhD; Murat Yarim, DVM, PhD; Ahmet Guler, PhD; Ahmet Ozak, DVM, PhD



In This Article


Subjective Observations

Signs of mild inflammation (redness and swelling) were observed immediately after wounding. However, these signs subsided within 1 week of wounding. None of the wounds displayed gross evidence of strong antigenic reaction to any of the three different types of honey (eg, edema, wound vesiculation, marked accumulation of exudate). None of the wounds (treated or control) displayed exuberant granulation tissue.

The honey-treated wounds were covered with a translucent layer of possibly nonabsorbed honey. This layer, however, was not observed in the control group.

A few micropustules were found in the neo-epidermis of the control wounds and even less were found in the honey-treated wounds. On day 7 there was no dehiscence, infection, or exudate seen on the surface of wounds in the honey-treated groups. The surfaces of the wounds were a pinkish color in three groups; however, the wounds in the control group were more swollen and warmer to the touch than those in the honey-treated groups. In the honey-treated groups, granulation tissue formation was easily noticeable at all wound edges on day 7. The median time for the first observable granulation tissue was not significantly different than that of the honey-treated groups and control group.

On day 14, an increased amount of epithelial tissue at the wound edges was observed in the honey-treated wounds in addition to a small elevation of granulation tissue at the center of the wound. Filling of the open wound to skin level with granulation tissue was significantly slower in the control group when compared to the other three groups. On day 17, three rabbits in the PBH group had complete coverage of the wounds with granulation tissue and epithelization, whereas, wounds in the other groups were not completely epithelialized. On day 21, the wounds of all treatment groups were almost completely epithelialized.