Nonviral Vectors for the Delivery of Small Interfering RNAs to the CNS

Inmaculada Posadas; Francisco Javier Guerra; Valentín Ceña

Disclosures

Nanomedicine. 2010;5(8):1219-1236. 

In This Article

Neural Transfection: Initial Approaches

Initially, the most commonly used techniques to introduce foreign DNA into eukaryotic cells were DNA/calcium phosphate coprecipitation and methods based on the use of high molecular weight (MW) compounds, such as dextran.[34,35] However, the use of these methods suffered from high variability in transfection efficiency, depending on the cell type transfected, and showed high toxicity in primary cultures. The maximum transfection efficiency achieved in mouse hypothalamic neurons was 3.5% for electroporation and rose to 6.5% after electroporation was combined with diethylaminoethyl-dextran.[16] Thus, other transfection strategies were developed in order to improve transfection efficiency and reduce toxicity, especially in primary neuronal cultured cells.

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