Effects of Occlusion on the Skin of Atopic Dermatitis Patients

Kristen Kobaly; Ally-Khan Somani; Thomas McCormick; Susan T. Nedorost


Dermatitis. 2010;21(5):255-261. 

In This Article


Transepidermal Water Loss

Table 1 shows the mean change in transepidermal water loss (TEWL) for the five subjects measured. TEWL was significantly increased by occlusion alone and to a greater extent by SLS under occlusion.

mRNA Expression

Cytokine mRNA data from tape stripping for individual subjects are shown in Table 2. At day 4, IL-8, IL-1α, and IL-1RA mRNA was induced by occlusion alone and SLS in all patients (with the exception of patient 6, who did not express IL-8 or IL-1α in response to occlusion). Expression of IL-8 mRNA under polyethylene occlusion alone was 2.17-fold greater than with SLS (p < .001) whereas expression of IL-1α under polyethylene occlusion was not significantly different when compared to SLS (see Table 1). Expression of IL-1RA was significantly lower under polyethylene occlusion than with SLS (a 0.56-fold change [p 5 .001]) (see Table 2).

Figure 1 shows the objective inflammation visible under occlusion and under the SLS patch site on the subject who had the most visible inflammation at the site of polyethylene occlusion alone. (Some subjects had no visible inflammation at this site.)

Figure 1.

Day-4 patch-test sites: top, site of sodium lauryl sulfate in three adjacent Finn Chambers; bottom, site of a square piece of polyethylene wrap. Both patch tests were removed at day 2.


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