Erythrocyte Sedimentation Rate Measurements by TEST 1 Better Reflect Inflammation Than Do Those by the Westergren Method in Patients With Malignancy, Autoimmune Disease, or Infection

Choong-Hwan Cha, MD; Chan-Jeoung Park, MD, Young Joo Cha, MD; Hyun Kyung Kim, MD; Duck Hee Kim; Honghoon; Jae Hoon Bae; Jae-Seol Jung; Seongsoo Jang, MD; Hyun-Sook Chi, MD; Dong Soon Lee, MD; Han-Ik Cho, MD


Am J Clin Pathol. 2009;131(2):189-194. 

In This Article

Materials and Methods

Blood samples anticoagulated with K3EDTA (Becton Dickinson, Franklin Lakes, NJ) were routinely obtained from hospitalized and ambulatory patients at the Asan Medical Center, Seoul, Korea, and processed for analysis. The concentration of K3EDTA was 1.8 mg/mL, and we used 3-mL EDTA tubes. All samples were obtained under standardized conditions (in the morning after a night of fasting) and tested within 4 hours of venipuncture, according to ICSH recommendations. Between October 2007 and November 2007, we selected 154 blood samples from patients with malignancy (n = 69), autoimmune disease (n = 44), or infection(n = 41); all patients had TEST 1 ESR values of 20 mm/h or more and hematocrit values between 33% and 35% (0.33–0.35) Table 1 .

Westergren Method

The Westergren method was performed according to ICSH specifications on undiluted blood samples anticoagulated with K3EDTA using glass pipettes (Greiner Bio-One, Kremsmuenster, Austria). During sedimentation, the pipettes were mounted vertically on appropriate supporting racks and kept at room temperature, which never exceeded 25°C.


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