The Effects of Lepidium sativum Seeds on Fracture-Induced Healing in Rabbits

Abdullah bin Habeeballah bin Abdullah Juma, FRCSEd

In This Article


This study, which lasted 12 weeks from the time of surgery, divided rabbits into control (no. = 3/C1, C2, C3) and test groups (no. = 3/T1, T2, T3). Soon after their recovery from general anesthesia, the control group was fed with a normal diet, but the test animals had a normal diet plus 6 g of L sativum seeds for the whole period of the study. The results of daily follow-up showed uneventful recovery, good healing of wounds, and weight gain in all rabbits. Documentation of fracture healing in the left femur of all groups was carried out and clinically based on the presence or absence of crepitus plus abnormal movements at the fracture sites, and radiologically from the site of callus formation. X-rays of the left femur at the fracture sites in all 6 rabbits were taken in a nearby hospital on 2 occasions -- the first at 6 weeks and the second at 12 weeks postoperatively. Both groups showed good healing of fractures and intact intramedullary K-wires (Figures 2 and 3).

Figure 2.

X-ray of control group at 6 weeks postoperatively.

Figure 3.

X-ray of test group at 6 weeks postoperatively.

The healing of fractures continued at 12 weeks and was almost complete in all groups (Figures 4 and 5).

Figure 4.

X-ray of control group at 12 weeks postoperatively.

Figure 5.

X-ray of test group at 12 weeks postoperatively.

Further documentation of the healing of fractures was carried out by a direct measurement, in millimeters, of the callus at the fracture sites LM, LL, and CM on the corresponding x-rays.

Data collected at 6 and 12 weeks postoperatively had callus formation measured at those sites and tabulated for further statistical analysis.

The SPSS statistical package was used to compare the test and control groups for any significant relationships or differences with regard to the healing of fractures in rabbits due to the consumption of L sativum seeds.

A statistically significant difference was found in one area (CM; P < .001) at 6 weeks of measurements of callus formation, as shown in Table 1 .

However, more areas revealed statistically significant differences at 12 weeks of measurements of callus formation in the LM and CM sites (P < .043 and P < .004, respectively; Table 1 ).

It was obvious that callus formation at 12 weeks continued to predominate in all areas of the test group and became more prominent in the LM and CM areas. This difference in callus formation between the test and control groups persisted at 6 and 12 weeks and was more evident in the test group.

Nevertheless, the weights of all rabbits, when documented weekly for 4 weeks, showed mild gradual increases in both groups with a slight tendency to be more evident in the test group, but this was not statistically significant ( Table 2 ).


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