The Effects of Lepidium sativum Seeds on Fracture-Induced Healing in Rabbits

Abdullah bin Habeeballah bin Abdullah Juma, FRCSEd

In This Article


The setting of the laboratory was sufficient to conduct this study under the direct operation and supervision of the author. Six adult New Zealand White rabbits (Oryctolagus cuniculus) of 6 months of age and weighing 4-5 kg were used and divided into 2 groups -- the control (C/no. = 3) and the test (T/no. = 3). Surgery was carried out under intramuscular general anesthesia with ketamine HCl 10-20 mg/kg body weight and xylazine 1-3 mg/10 kg body weight, exposing the midshaft of the left femur and inducing subperiosteal transverse fractures, which were then reduced and immobilized by intramedullary K-wires. All rabbits had uneventful recovery, were housed in cages, and were allowed to move freely without external support. Consequently, they were fed with a normal diet, but the test animals had, in addition, 6 g of L sativum seeds in their food daily. These seeds were obtained from the local market of the type grown in the Al-Qaseem area in Saudi Arabia from the L sativum plant (Figure 1).

Figure 1.

Lepidium sativum plant.

Follow-up of the diet, wound care, and behavior were carried out daily, and it was observed early the next morning that the rabbits were eating all of their food before adding 6 g of the seeds in their new food.

After 6 weeks postoperatively, all 6 rabbits (control and test) had their left femurs x-rayed in a nearby hospital. The study was continued as before and repeat x-rays in the same areas of the left femur were done at 12 weeks.

The study was ended and the animals were sacrificed followed by collection of data, tabulation, and statistical analysis with the SPSS package.

These data showed that the P values of the control and test groups at 6 weeks were P = NS, P = NS, and P < .001 in the longitudinal lateral (LL), longitudinal medial (LM), and circumferential (CM) callus measurements, respectively.

The P values at 12 weeks had more significant areas of P < .043 and P < .004 in the LM and CM callus, respectively. The LL callus remained nonsignificant (P = NS).


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