New Uses of Vinyl Tape for Reliable Collection and Diagnosis of Common Superficial Mycoses

Mario F.R. Miranda, MD, Ademir J.G. Silva


Skinmed. 2003;2(3) 

In This Article

Patients and Methods

Eighty-five patients (39 men, 46 women; age range, 1-93 years; mean, 28-76 years) presenting with scaly lesions clinically suspected of superficial mycoses were randomly included in the study. Only dry or, at most, slightly moist lesions were included; only one lesion was sampled per patient. The thin, clear, 19-mm wide Tapefix Adere (Campinas, São Paulo State, Brazil) adhesive tape was used in the experiments. The VAT strip was applied to the lesion, and a gentle pressure was applied with the bottom end of a test tube to ensure close adhesion of scales to the glue. The tape was removed from the lesion, and placed longitudinally, glue-face down, on a glass slide (preset with a drop of KOH/DMSO), thus providing a straight lining, and avoiding bubble formation with the glass surface. To do this, it is recommended to initially stick the edge of the tape to the corresponding glass edge, gently stretching the tape along the glass, and gradually laying it over the surface until the other edge can be completely set. In up to 5 minutes a complete clearing of the scales was obtained. The glass slide preparation was gently pressed against a filter paper to eliminate excess solution, then examined on the microscope under subdued lighting at 100-400 -range magnification, as usual. For each specimen yielding a negative result, a standard mount (i.e., scalpel sampled, KOH/DMSO cleared, cover glass) was also prepared as a corresponding control. Patients with proven negative results in both techniques have been considered a control group.


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