Langerhans Cell Granulomatosis Manifested as Pigmented Villonodular Synovitis

Cooley G. Pantazis, MD, Kimberly Templeton, MD, Ossama W. Tawfik, MD, PhD, Raul Braylan, MD

Disclosures

J South Orthop Assoc. 2001;10(4) 

In This Article

Immunohistochemistry

Immunohistochemical studies were done on deparaffined 5 mm sections with a robotic immunostainer (Ventana ES, Ventana Medical Systems, Tucson, Ariz) using the avidin-biotin complex. Reaction product was visualized by the chromogen diaminobenzidine. Monoclonal antibodies used in this study were CD1a, and S100 at 1:100 and 1:1600 dilution (Dako, Carpinteria, Calif). All sections were antigen retrieved using steam heating for 15 minutes in 0.01 M citric acid. Appropriate positive controls known to contain the antigens were processed at the same time.

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