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Figures for:
Down-Regulation of Pan-T-Cell Antigens, Particularly CD7, In Acute Infectious Mononucleosis

[Am J Clin Pathol 120(1):49-55, 2003. © 2003 American Society of Clinical Pathologists, Inc.]


Figure 1. An example of CD7 down-regulation in infectious mononucleosis by the CD8+ T-cell population, which is painted red. The T cells that do not show down-regulation of CD7 are green, the monocytes are blue, the granulocytes are gray, and CD45- debris is yellow. A, Moderate HLA-DR expression in the red population (arrow), as opposed to variable HLA-DR expression in the brighter CD7+ T cells (green). B, Down-regulation of CD7 and a slight increase in forward scatter (FSC) in the red population (arrow), as opposed to the brighter CD7+ T cells (green). Dual parameter dot plots of the mononuclear cell gate demonstrate CD7 down-regulation (red population) (C) and CD8 expression (D). APC, allophycocyanin; FITC, fluorescein isothiocyanate.

Figure 2. A, Dual-parameter display of the mononuclear cell gate shows down-regulation of CD7 by the CD8+ T-cell population (arrow) in another case. Note slight diminution of CD8 by the target cell population (red). B, T cells with CD7 down-regulation are CD4- (arrow). C, This same CD7 down-regulated T-cell population displays normal CD3 intensity (arrow). Red, CD8+ T-cell population; green, T cells that do not show down-regulation of CD7; blue, monocytes; gray, granulocytes; yellow, CD45- debris. APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

Figure 3. Down-regulation of CD5 (A) and CD7 (B) in infectious mononucleosis (arrows). Note the slight increase in forward scatter (FSC) in the aberrant population. Red, CD8+ T-cell population; green, T cells that do not show down-regulation of CD7; blue, monocytes; gray, granulocytes; yellow, CD45- debris. APC, allophycocyanin; FITC, fluorescein isothiocyanate.

Figure 4. Cytograms from an age-matched control patient. A and B, There is no increase in the forward scatter (FSC) or in any significant down-regulated CD7 population. There usually is a small "tail" of variably dim CD7 cells (B), but they are never a dominant or coherent population. C, There is a normal CD4/CD8 ratio. Red, CD7- to dim events; green, T cells that do not show down-regulation of CD7; blue, monocytes; gray, granulocytes. APC, allophycocyanin; FITC, fluorescein isothiocyanate.

Figure 5. Atypical lymphocytes in a peripheral blood sample in infectious mononucleosis (Wright-Giemsa, x1,000).