What is the role of high-performance liquid chromatography (HPLC) in the workup of vitamin toxicity?

Updated: Jun 01, 2020
  • Author: Mark Rosenbloom, MD, MBA; Chief Editor: Michael A Miller, MD  more...
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Johnson-Davis et al reported that a modified form of HPLC they developed shortened analysis time for serum concentrations of vitamins A and E. [47] Using their modifications—a high-throughput analytic column and small diameter tubing—to determine pediatric reference intervals for the 2 vitamins in 1136 healthy children, the authors found that their technique reduced run-time by 60%, mobile phase consumption by 39%, and sample injection volume by 50%.

A lumbar puncture may be indicated to rule out increased intracranial pressure in patients with vitamin A toxicity.

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