Which virus-specific lab studies are used for the diagnosis of human metapneumovirus (HMPV) pneumonia?

Updated: Mar 24, 2021
  • Author: Zab Mosenifar, MD, FACP, FCCP; Chief Editor: John J Oppenheimer, MD  more...
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Answer

It is difficult to isolate hMPV in standard cell cultures, and it replicates (grows) very slowly. The optimal cell line is tertiary cynomolgus monkey kidney or rhesus monkey kidney cell culture with trypsin added; cultures need to be observed for 21 days for cytopathic effect. Because of culture difficulties, RT-PCR is the preferred method for diagnosis.


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