How is the Affirm VPIII Microbial Identification Test used to diagnose trichomoniasis?

Updated: Jan 02, 2020
  • Author: Darvin Scott Smith, MD, MSc, DTM&H; Chief Editor: Michael Stuart Bronze, MD  more...
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Answer

The Affirm VPIII Microbial Identification Test detects the presence of Trichomonas, Gardnerella, and Candida species by using direct hybridization technology. Its sensitivity is 90%-100%, [79] and the detection threshold is reported to be 5,000 trichomonads/mL. Results from the Affirm VPIII test are available within about 45 minutes.

A bulletin on proper preparation and testing of specimens is available from the manufacturer. Specimens for which testing is expected to be delayed for more than 1 hour at ambient temperature or 4 hours with refrigeration should be stored in the Affirm VPIII Ambient Temperature Transport System (ATTS) for up to 72 hours. [80] In a study by Hollman et al, no difference was noted in detection of T vaginalis in urine samples compared with vaginal swabs. [81]


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