What is the role of molecular testing in the diagnosis of toxoplasmosis?

Updated: Mar 08, 2019
  • Author: Murat Hökelek, MD, PhD; Chief Editor: Michael Stuart Bronze, MD  more...
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Molecular diagnostic methods of diagnosing toxoplasmosis include techniques such as conventional polymerase chain reaction (PCR), nested PCR, and real-time PCR for detection of T gondii DNA in clinical samples. The original protocol for molecular detection of T gondii using con­ventional PCR targeted the B1 gene. Studies have also described detection of T gondii based on amplification of ITS-1 and 18S rDNA fragments, a method whose sensitiv­ity was similar to the B1 gene.

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