What is the role of blood culture in the diagnosis of Haemophilus influenzae infections?

Updated: Jul 02, 2019
  • Author: Joseph Adrian L Buensalido, MD; Chief Editor: Pranatharthi Haran Chandrasekar, MBBS, MD  more...
  • Print
Answer

Detection of the organism in a blood culture or any other body fluid is the most confirmatory method of establishing the diagnosis. Optimal growth requires the use of chocolate agar and BVCCA, which is a selective media of chocolate agar that contains bacitracin, vancomycin, and clindamycin. BVCCA has been found superior to chocolate agar for isolation of NTHi from nasopharyngeal swabs. [35] Some H influenzae strains grow best in 5%-10% carbon dioxide. [24]

The viability of H influenzae is lost rapidly, so clinical specimens should be inoculated to an appropriate culture media without delay. [24]

Seventy to 90% of patients with epiglottitis have positive blood culture results. However, to avoid laryngospasm, perform venipuncture and cultures of the inflamed epiglottitis only after the airway has been secured.


Did this answer your question?
Additional feedback? (Optional)
Thank you for your feedback!