How are stool specimens tested for cryptosporidiosis?

Updated: Nov 11, 2019
  • Author: Melinda B Tanabe, MD; Chief Editor: Michael Stuart Bronze, MD  more...
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Answer

Unconcentrated fresh specimens can be examined with wet mount preparations but are not recommended owing to high infectivity and unreliability. Despite different methods available, the formalin ethyl acetate concentration method is the most sensitive and widely used. [45] Optimal centrifugation time and speed are critical for concentrating Cryptosporidium oocysts. Commercial fecal concentration tubes are available that decrease processing time and supplies needed for concentrating specimens (eg, Fecal Parasite Concentrator, Evergreen Scientific). Immunomagnetic separation commercial kits are also available. Polyvinyl alcohol (PVA)–preserved specimens are not acceptable for modified acid-fast staining or antigen-detection assays for detection of Cryptosporidium.


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