What is the role of PCR in the diagnosis of brucellosis?

Updated: Apr 22, 2021
  • Author: Nicholas John Bennett, MBBCh, PhD, FAAP, MA(Cantab); Chief Editor: Michael Stuart Bronze, MD  more...
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Answer

Polymerase chain reaction (PCR) tests have been developed for the detection and rapid diagnosis of Brucella species in human blood specimens. [45] Two major genetic targets are the Brucella gene BCSP31 and the 16S-23S rRNA operon. The 16S-23S rRNA operon has been shown in studies to be more reliable in terms of sensitivity but is not yet widely used in clinical practice and needs more standardization. Possible applications would include evaluating cases of relapse and monitoring response to therapy.

Other promising tests include nested PCR, real-time PCR, [46, 47] and PCR-ELISA, but the clinical roles for all of these tests remain to be defined. [45, 48, 49, 50]


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