What is the role of cultures in the diagnosis of bartonellosis (Bartonella infection)?

Updated: Jun 19, 2019
  • Author: Kassem A Hammoud, MD; Chief Editor: Pranatharthi Haran Chandrasekar, MBBS, MD  more...
  • Print
Answer

Culture for Bartonella bacteria is not recommended for routine cases of patients with catscratch disease lymphadenopathy.

Cultures may be useful in patients who have other manifestations of either B henselae or B quintana infection, including fever of unknown origin, neuroretinitis, encephalitis, culture-negative endocarditis, and peliosis or bacillary angiomatosis. Fresh media is required to increase the chance of isolation.

A recent study suggests a novel chemically modified liquid medium that supports the growth of 7 Bartonella species. [36]

A 2016 study showed that a serum-free media using glucose, sucrose, and hemin as supplements was equivalent to the commercially available media but was easier to prepare. [37]

The lysis-centrifugation system (Isolator) is recommended for blood cultures.

Minced tissues may be cultured on chocolate agar plates in a humid atmosphere with carbon dioxide to facilitate growth.

Antibiotic susceptibility is not routinely tested in patients with bartonellosis because susceptibility studies may fail to predict response to therapy.


Did this answer your question?
Additional feedback? (Optional)
Thank you for your feedback!