What chemical stains can be used in the histological detection of Entamoeba histolytica in amebiasis?

Updated: Jul 19, 2019
  • Author: Vinod K Dhawan, MD, FACP, FRCPC, FIDSA; Chief Editor: Michael Stuart Bronze, MD  more...
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Answer

Different chemical stains can be used (see the image below), including periodic acid−Schiff stain, which makes E histolytica appear magenta in color.

Trichrome stain of Entamoeba histolytica cyst in a Trichrome stain of Entamoeba histolytica cyst in amebiasis. Each cyst has 4 nuclei with characteristically centrally located karyosomes. Cysts measure 12-15 mm.

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