Which techniques are used to detect amebiasis antibodies?

Updated: Jul 19, 2019
  • Author: Vinod K Dhawan, MD, FACP, FRCPC, FIDSA; Chief Editor: Michael Stuart Bronze, MD  more...
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Answer

Immunoelectrophoresis, counterimmunoelectrophoresis (CIE), and immunodiffusion (ID) use the precipitation property of antigen-antibody complexes in agar. CIE is time-consuming but has a sensitivity of 100% in invasive amebiasis. ID is simple to perform and thus ideal for laboratories that only rarely perform amebic serology; however, it requires a minimum of 24 hours, compared with 2 hours for IHA or ELISA. ID is slightly less sensitive than IHA and ELISA but is equally specific. Complement fixation (CF) is less sensitive than other techniques.

Although detection of immunoglobulin M (IgM) antibodies specific for E histolytica has been reported, sensitivity in patients with current invasive disease is only about 64%.


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