How are specimens collected and prepared for anticardiolipin and anti-?2-GPI antibodies detection?

Updated: Jul 22, 2021
  • Author: Tarek Hammad, MD; Chief Editor: Eric B Staros, MD  more...
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Answer

Answer

Obtain a 7-mL blood serum sample in a red-top tube.

Serum is preferred over plasma for testing.

Running samples in duplicate is recommended.

Polystyrene ELISA plates or high-sensitivity plates are preferred.

Cardiolipin should be diluted in ethanol. [5]

Control samples should be collected from at least 40 healthy young (< 50 years) people to determine the local cutoff for the above tests.


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