How are specimens collected and prepared for lupus anticoagulant detection?

Updated: Jul 22, 2021
  • Author: Tarek Hammad, MD; Chief Editor: Eric B Staros, MD  more...
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A blood serum sample is collected in a blue-top tube containing 0.109 M sodium citrate. Blood should be collected before the start of anticoagulation therapy or after a sufficient period following discontinuation.

Platelet-free (< 10,000/μL) plasma preparation via centrifugation must be done with special care, as it may cause platelet fragmentation and the release of phospholipids, which may lead to false-negative results.

Frozen plasma is required if testing is delayed, and frozen plasma must be thawed at 37°C. [1, 4]

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