How is von Willebrand factor (vWF) multimer analysis performed?

Updated: Aug 01, 2019
  • Author: Carlos Solano Loran, MD; Chief Editor: Eric B Staros, MD  more...
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Answer

Answer

Multimeric analysis of VWF is carried out by electrophoresis of plasma samples using nonreducing agarose gels in the presence of sodium dodecyl sulphate. [4]

Following electrophoresis, the following steps are required:

  1. The gels are fixed, washed, and dried and are then reacted with an antihuman vWF iodine I 125–labelled antibody.

  2. The specimens are Western blotted and then probed with enzyme-labeled antibodies for subsequent visualization of vWF multimers using enzyme substrates.

  3. Alternatively, enhanced chemiluminescence followed by autoradiography can be used to visualize the multimers.

The plasma vWF multimers analysis is a highly specialized, complex, and time consuming assay that requires specialized equipment and technical expertise but lacks standardization. [2]


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