What are the limitations of a factor V assay?

Updated: Feb 05, 2020
  • Author: Bishnu Prasad Devkota, MD, MHI, FRCS(Edin), FRCS(Glasg), FACP; Chief Editor: Eric B Staros, MD  more...
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Limitations of the test include partially clotted specimens due to poor mixture of anticoagulant (3:2 sodium citrate as per manufacturer’s blue topped tube), overfilled or underfilled test tubes altering the ratio of blood to anticoagulant (9:1), improperly stored plasma, contamination with heparin or dilution of collected sample if indwelling catheters are used or analytical errors such as lipemic, icteric, or hemolyzed plasma, which may interfere with photoelectric measuring instruments. [2]

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