How are the acidified serum lysis and the Ham test used in the workup of paroxysmal nocturnal hemoglobinuria?

Updated: May 20, 2021
  • Author: Emmanuel C Besa, MD; Chief Editor: Sara J Grethlein, MD, FACP  more...
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Answer

The serum pH is lowered to about 6.2 and the magnesium level is adjusted to 0.005 mol/L to achieve maximum sensitivity. The cells that are hemolyzed are the sensitive cells, and those that remain intact are normal cells, indicating 2-3 subpopulations of RBCs in the circulation.


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