What is the role of immunocytochemistry in the workup of mantle cell lymphoma (MCL)?

Updated: Mar 15, 2019
  • Author: Muhammad Rashid Abbasi, MD; Chief Editor: Emmanuel C Besa, MD  more...
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Answer

Answer

Tumor cells are monoclonal B cells that express surface immunoglobulin, immunoglobulin M, or immunoglobulin D. Cells are characteristically CD5+ and pan B-cell antigen positive (eg, CD19, CD20, CD22) but lack expression of CD10 and CD23. Cyclin D1 is overexpressed. Immunophenotyping helps differentiate MCL from other small B-cell lymphomas (see the Table, below). [5]

Table. Differential Diagnosis of Mantle Cell Lymphoma by Immunophenotyping (Open Table in a new window)

Disease

CD5

CD20

CD23

CD10

CD103

FMC7

Cyclin D1

Sig*

MCL

+

++

+/–

+

+

B-CLL/SLL

+

+

+

+

PLL

–/+

++

+/–

+

++

MZL§

++

+/–

+

SLVL||

++

–/+

+/–

++

LPL

+

–/+

++

FL#

++

+

+/–

++

HCL**

+

+

+

–/+

++

* SIg = surface immunoglobulins

B-CLL/SLL = B-cell chronic lymphocytic leukemia/small lymphocytic leukemia

PLL = prolymphocytic leukemia

§ MZL = marginal zone leukemia

|| SLVL = splenic lymphoma with villous lymphocytes

LPL = lymphoplasmacytic lymphoma

# FL = Follicular lymphoma

** HCL = Hairy cell leukemia


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