What is the role of flow cytometry analysis in the workup of NHL/B-cell lymphoma?

Updated: Feb 23, 2021
  • Author: Mohammad Muhsin Chisti, MD, FACP; Chief Editor: Emmanuel C Besa, MD  more...
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Answer

Flow cytometry analysis can help provide accurate diagnosis of some categories of B-cell lymphoma. Characteristic immunophenotypes are associated with major types of lymphoma (see the table below).

Table 2. Characteristic Immunophenotypes of Major Subtypes of Lymphoma (Open Table in a new window)

Lymphoma

Immunophenotype

Follicular

CD20+, CD3-, CD10+, CD5-, BCL2+, CD23-/+, CD43-, cyclinD-1-, BCL6+

Small lymphocytic

CD 20+, CD3-, CD10-, CD5+, CD23+,

MALT

CD20+, CD3-, CD 10-, CD5-, CD23-/+, BCL2+

Marginal zone

CD20+, CD3-, CD 10-, CD5-, CD23-/+, CD43-/+, cyclinD-1-, BCL2

Mantle cell

CD20+, CD23-/+, CD10-/+, CD5+, CD43-/+, cyclinD-1+

Mediastinal large B-cell

CD20+, CD30+, MUM-1+

Burkitt

slg+,CD20+, Tdt-, CD10+, BCL2--, BCL6+, Ki-67+ (≥95%)

B-lymphoblastic

slg-, CD19+, CD10-/+, CD20-/+, TdT+

MALT = mucosa-associated lymphoid tissue.


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