How is serologic crossmatching performed?

Updated: Jan 22, 2019
  • Author: Ashok Tholpady, MD, MSc; Chief Editor: Jun Teruya, MD, DSc, FCAP  more...
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An immediate spin phase is performed with donor RBCs prepared as a 2%-5% suspension in normal saline or ethylenediaminetetraacetic acid (EDTA) saline and the patient's serum.

Both samples are mixed together and centrifuged at room temperature to visualize hemolysis or agglutination, which would signify a positive test result and the presence of an additional antibody. Crossmatch-incompatible blood is rarely encountered, because donor blood units are selected after an effective antibody screen. When only the type and screen are combined with the immediate spin phase, this is known as an abbreviated crossmatch [4] and it is 99.9% effective in preventing an incompatible transfusion. [5]

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