How are negative direct antiglobulin test (DAT) results interpreted?

Updated: Jun 25, 2020
  • Author: Julie Katz Karp, MD; Chief Editor: Jun Teruya, MD, DSc, FCAP  more...
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Answer

Answer

The result is negative if no agglutination is observed at either test phase, but agglutination is observed after known IgG-coated and complement-coated RBCs are added to the appropriate tubes. [4]

In the case of a negative direct antiglobulin test, the known IgG-coated RBCs act as a positive control. If the known IgG-coated RBCs do not agglutinate, the test finding is presumed to be a false-negative result and must be repeated. [6]


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