What is the role of column agglutination in Rhesus (Rh) typing?

Updated: Aug 01, 2018
  • Author: Victoria K Gonsorcik, DO; Chief Editor: Jun Teruya, MD, DSc, FCAP  more...
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Answer

Answer

An individual's red cells are initially mixed in a small gel-filled tube that contains reagent anti-D. The specimen is centrifuged and assessed for agglutination. Agglutination seen at the top indicates a strong positive reaction; agglutination seen at the bottom signifies no reaction. The reaction is graded on a scale of 0 to 4+. See the image below.

Column agglutination "gel card." Image created by Column agglutination "gel card." Image created by Jaye Parsley.

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