What is the role of manual tube testing in Rhesus (Rh) typing?

Updated: Aug 01, 2018
  • Author: Victoria K Gonsorcik, DO; Chief Editor: Jun Teruya, MD, DSc, FCAP  more...
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Answer

Answer

An individual's red cells (in a 2%-5% saline suspension) are mixed in a test tube with anti-D reagent. The specimen is mixed gently. After centrifugation, the pelleted cell button is gently resuspended and examined for agglutination. Agglutination indicates a positive reaction. The reaction is graded on a scale of 0 to 4+. See the following image.

Manual tube testing. Image created by Jay Parsley. Manual tube testing. Image created by Jay Parsley.

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