What is the role of fixation in FFPE tissue testing of breast tumors?

Updated: Mar 20, 2019
  • Author: Oudai Hassan, MD; Chief Editor: Chandandeep Nagi, MD  more...
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Answer

Answer

When formalin-fixed paraffin-embedded (FFPE) tissue is used for testing, the importance of appropriate fixation cannot be overemphasized. The standard fixative is 10% neutral buffered formalin. Fixing within 1 hour of removal from the patient is recommended. Specimens should be sliced into 0.5- to 1-cm sections prior to fixing and placed in sufficient volumes of formalin. The duration of fixation should be kept between 6 and 72 hours. Sections for processing should be 2-4 mm thick.


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