What is the role of lab tests in the workup of low HDL cholesterol (hypoalphalipoproteinemia)?

Updated: May 21, 2021
  • Author: Vibhuti N Singh, MD, MPH, FACC, FSCAI; Chief Editor: George T Griffing, MD  more...
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See the list below:

  • Routine blood tests - Included among these is a chemistry profile.

  • Additional tests - These include liver function tests and a thyroid profile.

  • Plasma fasting lipid profile - After a 12-hour fast, plasma samples are obtained for lipid analysis. Total cholesterol and triglyceride (TG) levels are measured by enzymatic methods. The low-density lipoprotein (LDL) cholesterol level is determined in the supernatant after plasma precipitation with magnesium chloride–phosphotungstic acid. LDL cholesterol levels are estimated using the formula proposed by DeLong and colleagues. [25] Values obtained include LDL, HDL, total cholesterol, and TG levels.

  • Plasma lipid subfractions - Apo A-I, apo A-II, apo B, and lipoprotein Lp(a) are measured, using immunoassays for apo A-II and nephelometric assays employing antibodies for apo A-I, apo B, and Lp(a). Subfractions include apo A, apo A-I, apo A-II, apo A-III, apo B, apo C, and apo E.

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