What is the role of lab testing in the diagnosis of pemphigus vulgaris?

Updated: Sep 17, 2020
  • Author: Bassam Zeina, MD, PhD; Chief Editor: Dirk M Elston, MD  more...
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To establish a diagnosis of pemphigus vulgaris, perform the following tests:

  • Histopathology from the edge of a blister

  • Direct immunofluorescence (DIF) on normal-appearing perilesional skin [7]

  • Indirect immunofluorescence (IDIF) using the patient's serum if DIF results are positive: The preferred substrate for IDIF is monkey esophagus or salt-split normal human skin substrate.

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